ACCESS and DB_interface

Microplate well names and numbers in ACCESS software. Please note, the 48 well microplate well names differ from manufacturer standards!

Recommendations for initial OD, culture volume and sealing the plate

Find an experiment file and view the data.

Set a well as reference for all other wells in the growth plate, either before or after the experiment is completed

Use either the first well of each row or column as a reference for the rest of the row or column.

Convert data files from the Infinite reader to ACCESS data files using a custom HipScript. At present this is only available for 96 well format.

Data curves with problems such as contamination or bad data quality such as shaky curves can be flagged as use_data=no, so the data will not be used in large scale data retrieval and analysis. This can be done for an individual well or a whole plate.

The batch analysis feature of the DB_interface software allows users to define parameters, such as the reference well and a cutoff for the growth time, and apply these parameters to a list of growth plate files without having to open each individual growth file. Growth metrics are automatically recalculated using the new parameters and uploaded to the DB without further user intervention.

If there is a internet connection issue at the end of the run, the file did not automatically get uploaded to the server. Go to the ACCESS robot where the experiment was performed and make sure the file server is connected.

If a growth file has an incorrect name there is no need to stop the experiment and start over again. The file can be renamed from both the robot and DB interface software.

The following functions require an administrator password.

If there is a internet connection issue at the end of the run, the file did not automatically upload to the server.