Supplemental Table 3: Assessent of Indexing Tags
We computed the number of indexing tag with a perfect match, single mismatch, and two mismatches. We then determined the percentage of mismatches when compared to the perfect matches. Barcodes 97, 98 and 99 are not present in the experiment and are excluded from the analysis.# barcode id |
Barcode sequence |
0mm |
1mm |
2mm |
% increase by allowing 1mm |
% increase by allowing 2mm |
Note |
| bcB104_001 | GTGTAAGAGG | 2,473,356 | 289,386 | 371,416 | 11.7001 | 13.4437 | |
| bcB104_002 | AGGGAGTGGT | 1,714,039 | 200,576 | 231,278 | 11.702 | 12.0796 | |
| bcB104_003 | ATAGGTTATA | 1,850,379 | 186,148 | 238,455 | 10.06 | 11.7089 | |
| bcB104_004 | GGATGCGGTC | 2,068,661 | 165,659 | 245,831 | 8.00803 | 11.0025 | |
| bcB104_005 | GTGGTGTAAG | 1,435,785 | 199,356 | 245,527 | 13.8848 | 15.0156 | |
| bcB104_006 | GCGAGGGACA | 1,927,197 | 171,383 | 253,616 | 8.89286 | 12.0851 | |
| bcB104_007 | GGGTTATGCC | 2,638,999 | 303,038 | 402,045 | 11.4831 | 13.6655 | |
| bcB104_008 | GAGCGAGGAT | 3,174,804 | 397,311 | 554,232 | 12.5145 | 15.5155 | |
| bcB104_009 | AGGTTGCGAC | 2,175,828 | 171,153 | 226,778 | 7.86611 | 9.66254 | |
| bcB104_010 | GCGGTAAGCT | 2,434,451 | 330,295 | 432,109 | 13.5675 | 15.6292 | |
| bcB104_011 | GTGCGACACG | 1,789,855 | 149,565 | 188,871 | 8.35626 | 9.73853 | |
| bcB104_012 | AAGAGGAAAA | 1,603,374 | 133,101 | 202,476 | 8.30131 | 11.6602 | |
| bcB104_013 | GCGGTAAGGC | 2,212,324 | 295,164 | 418,728 | 13.3418 | 16.6991 | |
| bcB104_014 | GTGCGGCAGA | 2,231,850 | 203,986 | 287,884 | 9.13977 | 11.8187 | |
| bcB104_015 | GAGTTGAATG | 1,895,695 | 218,165 | 305,960 | 11.5084 | 14.474 | |
| bcB104_016 | GGGAGACGTT | 1,667,335 | 179,121 | 269,618 | 10.743 | 14.6019 | |
| bcB104_017 | GAGGTGGGAG | 2,619,648 | 294,960 | 406,986 | 11.2595 | 13.9637 | |
| bcB104_018 | GGTGGGACAC | 2,238,255 | 204,520 | 294,666 | 9.13748 | 12.0628 | |
| bcB104_019 | GTTATACTAA | 2,184,454 | 164,196 | 230,832 | 7.51657 | 9.82828 | |
| bcB104_020 | GCGAGATAAT | 1,629,383 | 182,283 | 153,690 | 11.1872 | 8.48335 | |
| bcB104_021 | GTGCGGTTCC | 2,338,971 | 229,244 | 360,558 | 9.80106 | 14.0392 | |
| bcB104_022 | GAGAAGATGA | 2,059,426 | 208,271 | 287,596 | 10.1131 | 12.6823 | |
| bcB104_023 | GCGGTGCTTG | 2,876,798 | 295,647 | 438,231 | 10.2769 | 13.8137 | |
| bcB104_024 | GGGTCGGTAT | 2,263,319 | 239,950 | 336,500 | 10.6017 | 13.4424 | |
| bcB104_025 | AGGGAGACAA | 1,951,215 | 223,693 | 285,012 | 11.4643 | 13.1046 | |
| bcB104_026 | GTGCATGGGT | 1,997,060 | 176,514 | 253,129 | 8.83869 | 11.6458 | |
| bcB104_027 | ATAGGTATAG | 2,221,465 | 238,916 | 336,923 | 10.7549 | 13.6939 | |
| bcB104_028 | GGACGGGAGC | 2,579,713 | 281,057 | 399,088 | 10.8949 | 13.9504 | |
| bcB104_029 | GAATCTAGGG | 1,889,163 | 174,295 | 225,554 | 9.22604 | 10.9309 | |
| bcB104_030 | GTAGCGAAGA | 2,277,144 | 259,035 | 372,724 | 11.3754 | 14.6963 | |
| bcB104_031 | GCTGGTGCGT | 2,549,675 | 208,803 | 320,146 | 8.1894 | 11.6059 | |
| bcB104_032 | GGTTGGGTGC | 2,711,481 | 229,483 | 331,196 | 8.46338 | 11.2615 | |
| bcB104_033 | GTAGTTGAAC | 2,921,140 | 271,828 | 385,371 | 9.30555 | 12.0694 | |
| bcB104_034 | AGTGCATTGG | 2,119,771 | 197,400 | 269,923 | 9.31233 | 11.6488 | |
| bcB104_035 | GCCGGAGTAT | 2,158,240 | 202,148 | 310,322 | 9.36634 | 13.1471 | |
| bcB104_036 | AAGGATTAGA | 1,933,757 | 200,184 | 285,668 | 10.3521 | 13.3869 | |
| bcB104_037 | AGGCTGTCTA | 2,382,742 | 207,739 | 269,538 | 8.71848 | 10.4049 | |
| bcB104_038 | GTGACCTACT | 1,678,193 | 141,585 | 186,754 | 8.43675 | 10.2625 | |
| bcB104_039 | GCGTATTGGG | 2,266,892 | 190,513 | 285,063 | 8.40415 | 11.6002 | |
| bcB104_040 | AAGGGATTAC | 2,658,664 | 240,043 | 358,691 | 9.02871 | 12.3742 | |
| bcB104_041 | GTTACGATGC | 2,160,558 | 163,404 | 243,193 | 7.56305 | 10.4646 | |
| bcB104_042 | ATGGGTGTTT | 2,798,428 | 164,210 | 230,876 | 5.86794 | 7.79292 | |
| bcB104_043 | GAGTCCGGCA | 2,451,033 | 187,671 | 253,395 | 7.65681 | 9.60301 | |
| bcB104_044 | AATCGAAGAG | 1,959,805 | 168,442 | 258,982 | 8.59483 | 12.1688 | |
| bcB104_045 | GTACTTGGCT | 2,865,645 | 223,248 | 323,206 | 7.7905 | 10.4635 | |
| bcB104_046 | GGTCGTCGAA | 2,602,068 | 204,844 | 283,770 | 7.87235 | 10.1097 | |
| bcB104_047 | GAGGGATGGC | 1,984,473 | 217,021 | 288,934 | 10.936 | 13.1245 | |
| bcB104_048 | GCCGTAAGTG | 2,490,320 | 225,649 | 338,510 | 9.06104 | 12.4637 | |
| bcB104_049 | ATGTCATAAG | 2,102,622 | 175,736 | 267,193 | 8.35795 | 11.7274 | |
| bcB104_050 | GAAGGCTTGC | 2,223,317 | 183,998 | 271,123 | 8.27583 | 11.2625 | |
| bcB104_051 | AAGCAGGAGT | 1,996,722 | 175,074 | 278,016 | 8.76807 | 12.8012 | |
| bcB104_052 | GTAATTGTAA | 1,985,153 | 149,343 | 220,446 | 7.523 | 10.3278 | |
| bcB104_053 | GTCATCAAGT | 2,956,629 | 264,804 | 405,655 | 8.95628 | 12.5924 | |
| bcB104_054 | AAAAGGCGGA | 2,299,795 | 210,804 | 254,777 | 9.16621 | 10.1481 | |
| bcB104_055 | AGCTTAAGCG | 2,413,422 | 355,880 | 302,285 | 14.7459 | 10.9156 | |
| bcB104_056 | GCATGTCACC | 2,754,022 | 197,242 | 176,831 | 7.16196 | 5.9917 | |
| bcB104_057 | CTAGTAAGAA | 2,167,276 | 172,294 | 276,036 | 7.9498 | 11.7986 | |
| bcB104_058 | TAAAGTGGCG | 1,956,698 | 130,595 | 153,255 | 6.67425 | 7.34228 | |
| bcB104_059 | AAGTAATGTC | 2,183,333 | 165,306 | 271,793 | 7.57127 | 11.5724 | |
| bcB104_060 | GTGCCTCGGT | 1,552,730 | 93,061 | 115,577 | 5.99338 | 7.02258 | |
| bcB104_061 | AAGATTATCG | 1,877,852 | 149,961 | 260,995 | 7.98577 | 12.8708 | |
| bcB104_062 | AGGTGAGGGT | 1,526,321 | 140,553 | 218,272 | 9.20861 | 13.0947 | |
| bcB104_063 | GCGGGTTCGA | 1,222,152 | 68,852 | 119,535 | 5.63367 | 9.25907 | |
| bcB104_064 | GTGCTACACC | 2,035,538 | 193,444 | 249,326 | 9.50334 | 11.1856 | |
| bcB104_065 | GGGATCAAGC | 2,365,076 | 263,579 | 374,844 | 11.1446 | 14.2599 | |
| bcB104_066 | GATGTAATGT | 2,198,976 | 210,025 | 316,410 | 9.55104 | 13.1345 | |
| bcB104_067 | GTCCTTAGGG | 1,984,563 | 123,823 | 191,227 | 6.23931 | 9.06983 | |
| bcB104_068 | GCATTGACGA | 1,820,830 | 167,349 | 249,814 | 9.19081 | 12.565 | |
| bcB104_069 | GATATGCTTT | 2,033,204 | 165,349 | 250,216 | 8.13244 | 11.3809 | |
| bcB104_070 | GCCCTACAGA | 1,819,933 | 143,207 | 195,675 | 7.86881 | 9.96745 | |
| bcB104_071 | ACAGGGAACG | 870,510 | 62,124 | 104,332 | 7.13651 | 11.1868 | |
| bcB104_072 | AAGTGAATAC | 1,926,349 | 152,353 | 268,080 | 7.9089 | 12.8965 | |
| bcB104_073 | GCAATGACGT | 2,105,002 | 178,087 | 250,840 | 8.46018 | 10.9869 | |
| bcB104_074 | AGGACGCTGA | 1,622,915 | 148,460 | 202,046 | 9.14774 | 11.4062 | |
| bcB104_075 | GTATCTGGGC | 1,708,025 | 130,939 | 233,126 | 7.66611 | 12.677 | |
| bcB104_076 | AAGTTTTAGG | 1,864,626 | 124,595 | 230,782 | 6.68204 | 11.6016 | |
| bcB104_077 | ATCTGGTCTT | 1,882,805 | 103,456 | 141,203 | 5.49478 | 7.10899 | |
| bcB104_078 | GGCAATCATC | 2,038,162 | 190,836 | 285,245 | 9.36314 | 12.797 | |
| bcB104_079 | AGTAGAATTA | 2,079,206 | 163,194 | 221,551 | 7.84886 | 9.88008 | |
| bcB104_080 | GTTTACGGTG | 1,734,693 | 125,997 | 173,813 | 7.26336 | 9.34132 | |
| bcB104_081 | GAACGTCATT | 2,014,615 | 164,711 | 252,308 | 8.17581 | 11.5773 | |
| bcB104_082 | GTGAAGGGAG | 3,106,346 | 344,210 | 470,437 | 11.0809 | 13.6337 | |
| bcB104_083 | GGATGGCGTA | 1,473,740 | 165,599 | 264,465 | 11.2366 | 16.1324 | |
| bcB104_084 | GCGGATGAAC | 1,845,246 | 211,465 | 320,526 | 11.46 | 15.5844 | |
| bcB104_085 | GGAAAGCGTT | 2,492,713 | 263,529 | 408,381 | 10.572 | 14.8166 | |
| bcB104_086 | AGTACCAGGA | 1,653,848 | 120,856 | 184,505 | 7.30756 | 10.3964 | |
| bcB104_087 | ATAGCAAAGC | 1,838,473 | 155,987 | 240,385 | 8.4846 | 12.0526 | |
| bcB104_088 | GTTGATCATG | 1,858,859 | 174,732 | 260,639 | 9.39996 | 12.8167 | |
| bcB104_089 | CTGTTATACC | 1,926,029 | 122,134 | 240,318 | 6.34123 | 11.7333 | |
| bcB104_090 | GTCGTCTTAG | 2,387,505 | 195,978 | 300,560 | 8.20849 | 11.6339 | |
| bcB104_091 | TATCGTGAGT | 1,869,914 | 163,996 | 253,260 | 8.77024 | 12.4519 | |
| bcB104_092 | AAAAGGGTTA | 2,120,262 | 175,972 | 280,086 | 8.29954 | 12.1976 | |
| bcB104_093 | TGTGGGATTG | 2,046,723 | 197,079 | 321,473 | 9.629 | 14.3272 | |
| bcB104_094 | GAATGTACTA | 2,056,587 | 212,318 | 291,410 | 10.3238 | 12.8436 | |
| bcB104_095 | CGCTAGGGTT | 2,273,812 | 194,392 | 297,040 | 8.54917 | 12.0347 | |
| bcB104_096 | AAGGATGATC | 1,614,876 | 136,312 | 228,986 | 8.44102 | 13.076 | |
| bcB104_097 | GAGTTTGTTT | 91 | 973 | 19,969 | X | X | Not in Pool |
| bcB104_098 | AATCGTTATG | 36 | 1,339 | 34,782 | X | X | Not in Pool |
| bcB104_099 | TGGGTAGGTC | 59 | 2,176 | 31,036 | X | X | Not in Pool |
| bcB104_100 | CGTAGATCTA | 494,829 | 264,073 | 181,536 | 53.3665 | 23.9209 | |
| bcB104_101 | CGTTGGATAC | 517,761 | 46,881 | 86,277 | 9.05456 | 15.2799 | |
| bcB104_102 | TCGTTAAAGG | 192,526 | 20,522 | 97,274 | 10.6593 | 45.6583 | |
| bcB104_103 | AAGCGTAGGA | 107,641 | 10,254 | 38,401 | 9.52611 | 32.5722 | |
| bcB104_104 | GTTCTCACAT | 745,601 | 51,613 | 92,771 | 6.92234 | 11.6369 |