Multiplexed Barcode Sequencing

Web supplement to
"Highly-multiplexed barcode sequencing: an efficient method for parallel analysis of pooled samples"

Andrew M Smith, Lawrence E Heisler, Robert P St.Onge, Eveline Farias-Hesson, Iain M Wallace, John Bodeau, Adam N. Harris, Kathleen M. Perry, Guri Giaever, Nader Pourmand, Corey Nislow

Nucleic Acids Research, doi:10.1093/nar/gkq368

Fig S1 : Structure of Amplicon used as Sequencing Template.

This schematic shows the structure of the PCR amplicon and depicts our method for sequencing both the yeast molecular barcode (First Read) followed by sequencing of the ABI SOLiD multiplexing tag (Second Read) which is used for sample discrimination.

 

 

Multiplexed Barcode SequencingInquiries can be addressed to guri.giaever@utoronto.ca OR corey.nislow@utoronto.ca