| TableS1 |
Re-characterization of the molecular barcodes
Presented here is a detailed breakdown for the up and down tags separately and the best tag. Paired-end (PE) represents data found in the Pair-End sequencing reaction and Single End (SE) in the Single End reads. PM represents the barcode that is a perfect match to the designed sequence while MM represents a mismatch of some type. This mismatch is further broken down in D1 (single deletion), S1 (single substitution), I1 (single insertion) and Multiple indicate multiple types of mutations present (i.e. two deletions).
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| TableS2 |
Re-characterization of universal priming sites for each barcode.
Presented here is a detailed breakdown for the common priming sites which flank the up and down tags separately, U1/U2 and D1/D2 respectively. PM represents the barcode was a perfect matched to the designed sequence while mismatches were further broken down in D1 (single deletion), S1 (single substitution), I1 (single insertion) and “Other” indicates multiple types of mutations present (i.e. two deletions).
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TableS3
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Validation of barcode recharacterization
Barcodes assigned to the uptag position for 35 yeast deletion strains were selected that had previously been assessed by Eason et al and which did not agree with our assessment. Recharacterization of these strains was based on identification of the barcode in both HT sequencing strategies (Paired-end with single-end confirmation, see methods). Our recharacterization indicated that 30 of the 35 barcodes had no alterations from the designed sequence. This was confirmed by a final round of Sanger sequencing for all 30. Our recharacterization indicated that 5 of the 35 barcodes differed from the designed sequence. The final round of Sanger sequence confirmed 4 of these 5 assessments. Characterizations that differ from the designed are indicated, with base substitutions, insertions or deletions highlighted in red.
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