Web supplement to
"Quantitative phenotyping via deep barcode sequencing"

Andrew M Smith, Lawrence E Heisler, Joseph Mellor, Fiona Kaper, Michael J Thompson, Mark Chee, Frederick P. Roth, Guri Giaever, Corey Nislow

Genome Research 2009, Oct:19(10):1836-1842. Epub 2009 Jul 21

Supplemental Table 2: Re-characterization of universal priming sites for each barcode.

Presented here is a detailed breakdown for the common priming sites which flank the up and down tags separately, U1/U2 and D1/D2 respectively. PM represents the barcode was a perfect matched to the designed sequence while mismatches were further broken down in D1 (single deletion), S1 (single substitution), I1 (single insertion) and “Other” indicates multiple types of mutations present (i.e. two deletions).

 

Inquiries can be addressed to guri.giaever@utoronto.ca OR corey.nislow@utoronto.ca