Web supplement to
"Quantitative phenotyping via deep barcode sequencing"

Andrew M Smith, Lawrence E Heisler, Joseph Mellor, Fiona Kaper, Michael J Thompson, Mark Chee, Frederick P. Roth, Guri Giaever, Corey Nislow

Genome Research 2009, Oct:19(10):1836-1842. Epub 2009 Jul 21

Recharacterization of barcodes and common priming sites

Barcodes and common priming sites in the Invitrogen Yeast Deletion Pool were recharacterized using high-throughput sequencing. The resequencing strategy, raw data files and recharacterized sequences are available below.

 

Barcode Recharacterization : Raw Sequencing Data
Sequencing Strategy
Raw fastq datafiles, compressed. Files are large (
Reaction Priming End Fastq File
Paired End - UPTAGS Barcode UP_ORF_read1_solexa.fastq
Paired End - UPTAGS Genomic UP_ORF_read2_solexa.fastq
Paired End - DOWNTAGS Barcode DN_ORF_read1_solexa.fastq
Paired End - DOWNTAGS Genomic DN_ORF_read2_solexa.fastq
Single End - UPTAGS Barcode UP_Kan_solexa.fastq
Single End - DOWNTAGS Barcode DN_Kan_solexa.fastq
     
Excel file indicating recharacterized Barcodes and Common Priming Sites
Barcode Recharacterization.xls    
     
     

 

 

 

Inquiries can be addressed to guri.giaever@utoronto.ca OR corey.nislow@utoronto.ca