Supplemental Table 3: Validation of barcode recharacterization
Barcodes assigned to the uptag position for 35 yeast deletion strains were selected that had previously been assessed by Eason et al and which did not agree with our assessment. Recharacterization of these strains was based on identification of the barcode in both HT sequencing strategies (Paired-end with single-end confirmation, see methods). Our recharacterization indicated that 30 of the 35 barcodes had no alterations from the designed sequence. This was confirmed by a final round of Sanger sequencing for all 30. Our recharacterization indicated that 5 of the 35 barcodes differed from the designed sequence. The final round of Sanger sequence confirmed 4 of these 5 assessments. Characterizations that differ from the designed are indicated, with base substitutions, insertions or deletions highlighted in red.
