Genome-wide Screen in S. Cerevisiae

Web supplement to
"Genome-wide screen in Saccharomyces cerevisiae identifies vacuolar protein sorting, autophagy, biosynthetic, and tRNA methylation genes involved in life span regulation"

Paola Fabrizio, Shawn Hoon, Mehrnaz Shamalnasab, Abdulaye Galbani, Min Wei, Guri Giaever, Corey Nislow, and Valter D. Longo

tbd

Supplementary Tables (Excel Workbooks)

TableS1 CFUs values relative to the survival of the two YKO pools used for the genome-wide screen.
TableS2 Mutant-specific aging profiles were obtained by calculating the log2 ratios for each time point using day 3 as a reference (see Methods). Data from both biological replicates and their average are shown. The root squared mean error (RSME) between the two replicates is also shown. The last column on the right indicates whether the corresponding mutants were included in the K-means clustering analysis.
TableS3 The CFUs for each strain for pool 1 and 2 at each time point was estimated by multiplying the microarray fold-ratio for each strain (table S2) by the CFUs number for the pools at the corresponding time point (table S1). The average number of CFUs was estimated by averaging between the two pools.
TableS4 Deletion mutants were ranked by p and q values obtained by EDGE analysis.
TableS5 p and q values obtained by EDGE analysis for the mutants classified as short-lived by K-mean clustering
TableS6 p and q values obtained by EDGE analysis for the mutants classified as long-lived by K-mean clustering.
TableS7 Gene ontology analysis of the short-lived mutants identified by K-means clustering. Significantly enriched categories (p<0.01) are enlisted.
   

 


Inquiries can be addressed to guri.giaever@utoronto.ca OR corey.nislow@utoronto.ca