Supplementary Figures
Figure | Title |
Figure S1 | CLS of wild type BY4741 and cup9Δ mutants switched to water on day 3. A representative experiment is shown. |
Figure S2 | CLS of BY4741 and acb1Δ transformed with either control vector or a centromeric plasmid carrying the ACB1 gene driven by its on promoter. Yeast strains were grown in either SDC or in selective SDC-uracil and transferred to water on day 3. A representative experiment performed in triplicate is shown. |
Figure S3 | A) Heat-shock resistance of day 3 DBY746 cultures treated with TBBt (5-15 μM) on day 2. B) CLS of DBY746 cells treated with TBBt on day 2 and 5. DMSO was used as a vehicle. A representative experiment is shown. |
Figure S4 | CLS of putative long-lived mutants identified by genome-wide screen. A) CLS of wild type (BY4741) and mutants lacking Far3, Far11, Pan2, or Bul1 transferred to water on day 3. A representative experiment performed in triplicate is shown. B) CLS of wild type and of a ppg1Δ mutant under starvation/extreme CR. The average of two independent experiments performed in duplicate is shown. |