Description is as in Figure 4. All negative results with the Magic Marker strains (top round panels) were confirmed by tetrad dissection (representative tetrads are in bottom rectangular panels, if applicable).
Top panel (round plates): left, negative control (vector-only); center, complementation with C. albicans ORF; right, positive control (complementation with corresponding S. cerevisiae ORF).

Bottom panels (rectangular): all tetrads were replica-plated onto media containing 5-fluoroorotic acid (5-FOA) or geneticin (G418) to confirm that the overexpression clone was the source of complementation. First row: the tetrad dissection (left is negative control (vector-only); center, complementation with C. albicans ORF; right, positive control (complementation with corresponding S. cerevisiae ORF)).
Second row: replica plate to 5-FOA; third row: replica plate to geneticin (G418).