(A) Transposon insertion sites are plotted by coordinate organized by chromosome. We used one commercial library (blue) and constructed a total of six genomic libraries. Green: genomic DNA was digested to completion with XbaI, yellow: SpeI, black/cyan: genomic DNA was partially digested with XbaI/SpeI or XbaI/EcoRV; red: remaining libraries were generated by digestion of genomic DNA with either EcoRV or BsrBI. The majority of mutants were generated using either XbaI- or SpeI-generated genomic libraries.

(B) Close-up of terminal region of chromosome 8; colors are as in (A).