Web supplement to
"BarSeq : Identification and Counting of Short Molecular Barcodes in HT-Seq data"

Supplementary Files

The barseq analysis is available as either

• An executable, compiled under various distributions (and available as source code written in C)
.
• A perl script

There are small differences in the two approaches. The current implementation is as a binary. The perl script is still being made available but will eventually be removed.

File	Title	Description
barseq_ubuntu12	Binary, Linux	Compiled under Ubuntu Linux 12
barseq_OSX_10.5	Binary, OSX	Compiled under OSX 10.5
barseq_win7.exe	Binary, Windows	Compiled under Windows 7
barseq_source.zip	Binary, C Source Files	for compilation under other OS
barseq.zip	Perl Scripts	barseq.pl : main barseq analysis script; barseq.pm : perl module with required functions
HET Pool Barcodes	Barcode file, for the HET Pool	The Collection of Barcodes for the Heterozygous Yeast Deletion Pool. This dataset contains sequences for both the uptag and downtag barcodes. Sequences identifiers are the Yeast Deletion strain and the associated tag position (up/dn). Sequence orientation matches the expected sequences generated based on the protocol used.
het_test_qseq.txt	Sample input file, qseq format	Illumina qseq file generate from ht-seq of a sample containing strains in the Heterozygous Yeast Deletion Pool
Sample output files	Sample Output Files	Files generated along each step along the pipeline qseq/fastq -> readcounts -> clusters -> map -> mappedbarcodes