Web supplement to
"Functional Analysis with a Barcoder Yeast Gene Overexpression System"

Supplementary Figure S2 : Universal primers (A) and strategy (B) used for the construction of barcoder strains.

UPTAG and DOWNTAG primer pairs used to amplify the kanamycin resistance cassette with 20-mer barcodes are shown. Primers U2 and D1 are homologous to the kanMX cassette, and U1 and D2 are homologous to the ho locus. PCR reactions were performed using Platinum PCR Supermix High Fidelity (Invitrogen). The transformants were selected by replica-plating onto standard yeast peptone dextrose (YPD) + G418.